Proteoform Thursday: Emily Byrd presents: “30 nm Diameter Nanopipette nESI Emitters Significantly Enhances Desalting and Sensitivity for Native Mass Spectrometry of Large Proteins”

April 23, 2026 11:00 AM in Eastern Time (US and Canada)

Sub-50 nm quartz nanopipette nanoESI emitters provide a simple route to extend native MS and native top-down workflows into physiological buffers and complex mixtures by suppressing salt adduction. Benchmarking across a Waters Synapt G2-Si and Thermo Fisher Orbitrap platforms (UHMR and Ascend), spanning proteins and assemblies from 17 to 800 kDa, demonstrates that gentler source conditions improve preservation of noncovalent complexes and metalloproteoforms. Direct measurements from phosphate buffers and crude bacterial lysates expressing α-synuclein further demonstrate matrix tolerance without laborious offline cleanup. Together, these advances lower practical barriers to native and top-down proteoform analysis under biologically relevant conditions and support routine application in complex samples.