Proteoform Thursday: Tarick El-Baba presents “Isolation of Metabotropic Glutamate Receptors from Human Brain Uncovers Remodeling in Depression”

December 12, 2024 11:00 AM in Eastern Time (US and Canada)

Metabotropic glutamate receptor 2 (mGluR2) is a class C GPCR that requires dimerization to function as a glutamate autoreceptor in the brain. I will describe an approach to enrich mGluR2 and other membrane protein transporters from minute quantities of human and mouse brain tissue. By integrating native MS and native top-down MS, I will describe how we have observed multiple alterations to mGluR2 interactions that are associated with the expression of pro-depressive behaviors in mice and with major depressive disorder in humans. Taken together, this provides direct evidence for maladaptive glutamate receptors in MDD.