Intact Proteoform Analysis by Capillary Electrophoresis–Mass Spectrometry. Are We There Yet?
The Consortium Initiative to develop guidelines for the use of online capillary electrophoresis in separating proteins and non-covalent protein complexes for top-down and native mass spectrometry has finalized their work and published the findings. For the first time, research teams from around the world validated the robustness, repeatability, and reproducibility of CZE-MS for TDP in both simple and complex model proteoform mixtures employing a full spectrum of commercially available capillary electrophoresis-MS interfaces. The study was led by Liangliang Sun of Michigan State University, Alexander R. Ivanov of Northeastern University, and Kevin Jooss of the Vrije Universiteit Amsterdam.
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